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All cells were grown in medium supplemented with 1% L-Glutamine (Thermo Fisher Scientific) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific) at 37 C in a humidified atmosphere of 5% CO 2

Red flag symptoms requiring urgent evaluation include sudden severe pelvic/abdominal pain, fever, heavy bleeding with syncope, positive pregnancy test (to rule out ectopic pregnancy), or signs of bowel or urinary obstruction

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[DOI] [PMC free article] [PubMed] [Google Scholar] 100.Thanapirom K., Suksawatamnuay S., Tanpowpong N., Chaopathomkul B., Sriphoosanaphan S., Thaimai P., Srisoonthorn N., Treeprasertsuk S., Komolmit P